96 Wells Mouse Anti-nuclear Antibody ELISA Kit High Sensitive for Research Use

96 Wells Mouse Anti-nuclear Antibody ELISA K High Sensitive for Research Use

Cat.No E0253Mo

Standard Curve Range: 1pg/ml - 400pg/ml

Sensitivity: 0.53pg/ml

Size: 96 wells

Assay Principle

This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse ANA antibody. ANA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse ANA Antibody is added and binds to ANA in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ANA antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse ANA. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Reagent Provided

Material Required But Not Supplied

• 37°C±0.5°C incubator
• Absorbent paper
• Precision pipettes and disposable pipette tips
• Clean tubes
• Deionized or distilled water
• Microplate reader with 450 ± 10nm wavelength filter

Reagent Preparation

All reagents should be brought to room temperature before use.

Standard Reconstitute the 120μl of the standard (480pg/ml) with 120μl of standard diluent to generate a 240pg/ml standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (240pg/ml) 1:2 with standard diluent to produce 120pg/ml, 60pg/ml, 30pg/ml and 15pg/ml solutions. Standard diluent serves as the zero standard(0 pg/ml). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:

Wash Buffer Dilute 20ml of Wash Buffer Concentrate 30x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.

sandwich elisa kit

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96 Wells Mouse Anti-nuclear Antibody ELISA Kit High Sensitive for Research Use Images